Fig. 4

Illustration of various algorithms performance using clustering method for evaluating over-merging/over-splitting based OTU_ref. (A) stacked bar plots represent total number of reference sequences for single-end and paired-end conditions for Mockrobiota and HC227_V3V4 datasets respectively. B) Average box plots representing the correct percentage for single-end and paired-end reads for Mockrobiota community C) Average box plots representing the over-splitting percentage for single-end and paired-end reads for Mockrobiota community, D) Average box plots representing the over-merging percentage for single-end and paired-end reads for Mockrobiota community