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Fig. 2 | Environmental Microbiome

Fig. 2

From: Peptide nucleic acid (PNA) clamps reduce amplification of host chloroplast and mitochondria rRNA gene sequences and increase detected diversity in 16S rRNA gene profiling analysis of oak-associated microbiota

Fig. 2

Bacterial diversity and community profiles in oak tissue samples following 16S rRNA gene amplification with and without PNA clamps. All reads assigned to host chloroplast and mitochondria were removed before analysis. A. Shannon diversity index. Stars indicate significant differences (Wilcoxon rank sum test. Leaf: p-value = 0.0087; Bark: p-value = 0.0022, Roots; p-value = 0.94) B. Bray-Curtis Non-metric multidimensional scaling (NMDS). Solid (no PNA clamps) and dashed (pPNA + qmPNA clamps) ellipses represent 95% confidence intervals of normal multivariate distributions. C. Phyla relative abundance based on 16S rRNA gene reads. The top 6 most abundant phyla are presented, and the remaining phyla are categorised into Other (grey). Each bar represents one tissue samples from one tree. For each tissue type the first three bars represent Q. petraea from the Little Snoring site, while the last three bars represent Q. robur from three different sites

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Environmental Microbiome

ISSN: 2524-6372