Methane-cycling microbial communities from Amazon floodplains and upland forests respond differently to simulated climate change scenarios

Table 1 Set of primers and references for each gene used in this study

Gene	Objective	Target group	Primers	References
16S rRNA	Amplicon Sequencing	Bacteria and Archaea	515F	Parada et al. [68]
16S rRNA	Amplicon Sequencing	Bacteria and Archaea	806R	Apprill et al. [3]
16S rRNA	qPCR	Total archaeal community	519F	Klindworth et al. [39]
16S rRNA	qPCR	Total archaeal community	915R	Stahl and Amman [78]
16S rRNA	qPCR	Total bacterial community	515F	Caporaso et al. [8]
16S rRNA	qPCR	Total bacterial community	806R	Caporaso et al. [8]
mcrA	qPCR	Methanogenic Archaea	mlas-mod-F	Steinberg and Regan [79]
mcrA	qPCR	Methanogenic Archaea	mcrA-rev	Steinberg and Regan [79]
pmoA	qPCR	Methanotrophic Bacteria	A189f	Holmes et al. [33]
pmoA	qPCR	Methanotrophic Bacteria	MB661r	Costello and Lidstrom [11]

ISSN: 2524-6372