Fig. 9

Comparison of seasonal microbiomes on lettuce cultivars stored in MAP. a NMDS plots of bacterial community composition by cultivar on processed lettuce pre-storage and with storage at 6 °C (mid- and endpoint included). P values for differences in the spring (green) and fall (brown) metagenomes were computed using PERMANOVA and included in the plots. Samples are further categorized as from conventional (C, circles) and experimental (E, squares) fields. b Average relative abundance (RA) of bacterial species in lettuce metagenomes by cultivar and season with storage at 6 °C. Species with RA ≥ 0.1 for at least one cultivar and season combination are listed and identified taxa with lower RA are included in ‘other taxa’. c Table listing the ratio of relative abundance of Pseudomonadaceae to Erwiniaceae in the average metagenomes by lettuce cultivar and season with storage at 6 °C. d Histogram of LDA scores computed using LEfSe analysis for taxa that are differentially abundant in spring (green) and fall (brown) microbiomes with storage at 6 °C (biomarkers with LDA value ≥ 3.0 depicted). P. simiae was found to be a poor biomarker upon closer examination of the RA results